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Fig. 3 | BMC Oral Health

Fig. 3

From: Nicotine regulates autophagy of human periodontal ligament cells through α7 nAchR that promotes secretion of inflammatory factors IL-1β and IL-8

Fig. 3

Effects of nicotine and/or 3-MA on the expression of autophagy-related markers in hPDLCs. A LC3II and Beclin-1 expression in hPDLCs assessed by Western blot analysis; B LC3II protein quantitative analysis. Data were expressed as mean ± SD from at least three independent experiments; C Beclin-1 protein quantitative analysis. Data were expressed as mean ± SD from at least three independent experiments; D TEM was used to evaluate autophagy induced by nicotine (10−5 mol/L, 12 h) and/or α-BTX (10−8 mol/L, 12.5 h). Magnification, 40,000×. Scale bar, 0.5 µm. The red arrows represent autolysosome; E Quantitative analysis of autolysosomes in one cell, at least 3 cells in each group for statistics (*p < 0.05, **p < 0.005, ***p < 0.001)

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