Fig. 3

Representative CLSM images of live or dead E. faecalis biofilms on canal walls in the middle region of root canals after irrigation. A The left and middle-left images of each row represent 2D and 3D structures of biofilms in the middle region irrigated by conventional needle irrigation (CNI). The right and middle-right images represent 2D and 3D structures of biofilms on in the middle region irrigated by photon-induced photoacoustic streaming (PIPS). B The percentages of dead bacteria in E. faecalis biofilms are shown. The values are shown as mean and standard deviation. Statistical significance is represented by uppercase letters