Fig. 7
From: RNAseq of TGF-β receptor type I kinase-dependent genes in oral fibroblast exposed to milk
Smad2/3 immunostaining of gingival fibroblasts exposed to milk. Serum-starved gingival fibroblasts were exposed to 5% of an aqueous fraction of human milk (HM) with and without 10 µM SB431542, 10 μM LY2157299 and 40 µM D609 for 30 min before fluorescent labelling of Smad2/3. The nuclear signal is visible with cells exposed to human milk (HM) and blocked by SB431542, LY2157299 but not by D609. The characteristic nuclear signal is indicated by demarcation of the relatively stronger green color towards the faintly stained cytoplasm. Scale bar indicates 100 µm. ”wo” stands for without, indicating the unstimulated cells