Fig. 4

Knockdown FERMT1 inhibited EMT and the PI3K/AKT signaling pathway. OSCC cells were treated with 5 ng/ml recombinant human TGF-β1 protein. (A, B) mRNA Expression (A) and protein levels (B) of mesenchymal biomarkers including vimentin, N-cadherin, and MMP-9, epithelial biomarkers including E-cadherin, and PI3K, total (t)-AKT, and phosphorylated (p)-AKT were measured by qRT-PCR and western blotting after transfection in Tca8113 and SCC15 cells at 24 h. (C) The representative image of western blot