Table 1 RT-PCR and real-time PCR primer
From: Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study
Primer | Sequence |
|---|---|
RT-PCR | Â |
18s rRNA+ | 5′-GGGGATCGAAGATGATCAGA |
18s rRNA− | 5′-CACGACGGAGTTCACAAGA |
HGC1+ | 5′-CATTAACTCCAAAATCAATTTCACAACA |
HGC1− | 5′-ATCGAGTTTTAGTATA A ATAGGAGA |
EFG1+ | 5′-ACGGAAATTACAATAACGGTATGCCC |
EFG1− | 5′-TTCTTTGGCAACAGTGCTAGCTGAT |
ECE1+ | 5′-ATTCTCCAAAATTGCCTGTGCTA |
ECE1− | 5′-AGCTTTTCCGA A ATATTCTTCAATC |
ALS3+ | 5′-TGTTACTCATATATTTGTCGGTTGC |
ALS3− | 5′-ACATGGTGTCATAAATAATCACAG |
HWP1+ | 5′-ACTGCTCAACTTATTGCTATCGCTT |
HWP1− | 5′-TGTTACCAGCACCTTCAAAGTAGA |
Real-time PCR | Â |
18s rRNA+ | 5′-TCTTTCTTGATTTTGTGGGTGG |
18s rRNA− | 5′-TCGATA GTCCCTCTAAGAAGTG |
HGC1+ | 5′-GGTAACACCACCAAATC |
HGC1− | 5′-GAAGAAACAGCACGAGA |
EFG1+ | 5′-TTCTGCTTCGGCTCCTC |
EFG1− | 5′-CTGCTGTTGGTTGTAAGTTTGA |
ECE1+ | 5′-GGCAACATTCCACAAGTA |
ECE1− | 5′-AGGACGCCATCAAAAA |
ALS3+ | 5′-GTGACCACCTTACCATTCG |
ALS3− | 5′-ACAGTAGCAGTTTCCCCAAT |
HWP1+ | 5′-CGGAATCTAGTGCTGTCGTCTCT |
HWP1− | 5′-TAGGAGCGACACTTGAGTAATTGG |