Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women

Table 1 Primer sequences used in real-time PCR

Bacteria (16S rRNA)	Primer sequences (5′-3′)	Reference
Bifidobacterium
F	CTCCTGGAAACGGGTGG	[17]
R	GGTGTTCTTCCCGATATCTACA	[17]
B. adolescentis
F	GGATCGGCTGGAGCTTGCTCCG	[19]
R	CCCCGAAGGCTTGCTCCCAGT
P	[FAM]CTCCAGTTGGATGCATGTCCTTCTGG[TAM]
B. dentium
F	ATCCCGGGGGTTCGCCTCC	[19]
R	ATACCGATGGAACCTTTCCCGG
P	[FAM]TGCTCCGGTTGGATGCATGTCCTTCC[TAM]
P. gingivalis
F	AGGCAGCTTGCCATACTGCG	[15]
R	ACTGTTAGCAACTACCGATGT	[15]
P. intermedia
F	TTTGTTGGGGAGTAAAGCGGG	[18]
R	TCAACATCTCTGTATCCTGCGT	[18]
A. actinomycetemcomitans
F	CTTACCTACTCTTGACATCCGAA	[16]
R	ATGCAGCACCTGTCTCAAAGC	[16]

ISSN: 1472-6831