Fig. 3
Expression of HIF-1α and PCNA induced by miRNA-18a in OSCC under hypoxic conditions. mRNA expression of A miRNA-18a and B HIF-1α induced by miRNA-18a mimics in OSCC cell lines according to qRT-PCR analysis. U6 was used as the control gene. C Protein expression of HIF-1α and PCNA induced by C miR-18a mimics or D miRNA-18a and CoCl2 in OSCC cell lines according to western blot analysis. Intensity graph showing the expression level of proteins after treatment with C miR-18a mimics or D miRNA-18a and CoCl2 in OSCC cell lines, as determined with western blotting. *Significant difference in expression between control- and miRNA-18a-treated or miRNA-18a and CoCl2-treated OSCC cell lines (p < 0.05). GAPDH was used as the control gene. All experiments were performed at least in triplicate. miR-18a, microRNA-18a; CoCl2, cobalt chloride. The original pictures of western blots of C and D were shown in Additional file 2: Fig. S2, S3 and Fig. S4, S5.