Fig. 5
Cell viability, migration, and invasion abilities of OSCC cell lines under hypoxic conditions with miRNA-18a treatment. A Representative images from the EdU assay in which YD-10B and HSC-2 cells were treated with CoCl2 and miR-18a or miRNA-18a alone (scale bar: 200 μm; original magnification: × 10). B Graphs showing the mean positive cell number for proliferation in five random regions in each well in the two tested OSCC cell lines. C Graphs showing cell viability, as measured by an MTT assay in YD-10B and HSC-2 cells. Representative images of D migration ability (scale bar: 200 μm; original magnification: × 10) and E invasion ability (scale bar: 200 μm; original magnification: × 20) in YD-10B and HSC-2 cells under CoCl2 treatment induced with miRNA-18a treatment. Graphs showing the mean number of migrated and invaded cells induced by miRNA-18a treatment in YD-10B and HSC-2 cells under hypoxic conditions (bottom panels). These values were obtained from five random regions in each well. *Significant difference between cells treated with miRNA-18a alone and those treated with miRNA-18a under hypoxic conditions (p < 0.05). miR-18a, microRNA 18a; CoCl2, cobalt chloride