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Table 2 Antibody details and incubation protocols

From: Characterization of immunologically detectable T-cell sensitization, Immunohistochemical detection of pro-inflammatory cytokines, and clinical parameters of patients after allogeneic intraoral bone grafting procedures: a prospective randomized controlled clinical trial in humans

Antibody

Isotype

Manufacturer

Incubation protocol

CD4

rabbit monoclonal

Abcam (Cambridge, UK)

HP, EDTA buffer, 1:50, o/n, RT

CD8

mouse monoclonal

Dako (Glostrup, Denmark)

HP, citrate buffer, 1:50, 1 h, RT

IL-1α

goat polyclonal

Santa Cruz (Dallas, USA)

1:50, o/n, 4 °C

IL-1ß

rabbit polyclonal

Abcam (Cambridge, UK)

1:100, o/n, 4 °C

TNF-α

mouse monoclonal

Santa Cruz (Dallas, USA)

1:100, o/n, 4 °C

  1. HP Heat pretreatment, o/n overnight, RT Room temperature
  2. Table 2 provides an overview of the antibodies used for immunohistochemical staining regarding isotype, manufacturer, and incubation protocols