Fig. 3

The expression of BACH1 was changed during the odontoblastic differentiation of hDPSCs. a Changes in the mRNA level of BACH1, DMP1 and DSPP on OM days 0,1,3,7 and 14. GAPDH was used as an internal control. b Changes in the total protein level of BACH1, DMP1 and DSPP on OM days 0, 1, 3, 7 and 14. Quantification of the relative protein expression via Image Pro Plus software, in which GAPDH was served as the internal control. c BACH1 protein expression of nuclear fraction on OM days 0, 1, 3, 7 and 14 had been tested by Western blot analysis. Lamin B1 was used as loading control. d Representative immunofluorescent labelling images are displayed. BACH1 (red), DAPI (blue) and Phalloidin (green) were imaged to show the subcellular distribution of BACH1 on OM days 0, 1 and 14. Scale bar = 10 µm. Data were presented as the mean ± standard error of the mean (n = 3). Statistically significant compared to the day 0 group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The gels in b, c were cropped, full-length gels are presented in Additional file 1: Original Band of Western blot Analysis Figs. S1 and S2. In Fig. 3b, the bands of BACH1 and GAPDH were from the same gel, while the bands of DSPP and DMP1 were from different gels. In Fig. 3c, the bands of BACH1 and Lamin B1 were from the same gel