Fig. 5

BACH1 affected the odontoblastic differentiation of hDPSCs in vitro. a Transduced hDPSCs were cultured with OM for 7 days. ALP activity was evaluated by ALP staining and ALP activity assay in the LV-NC and LV-shBACH1 group. b hDPSCs were cultured with OM for 21 days. Calcium nodule deposition was evaluated by ARS staining assay. Scale bar = 200 µm. c mRNA level of BACH1, HMOX-1 and odontogenic markers in LV-NC and LV-shBACH1 hDPSCs on OM 14 days. GAPDH was used as an internal control. d Total protein level of BACH1, HO-1 and odontogenic markers in LV-NC and LV-shBACH1 hDPSCs on OM 14 days. Quantification of the relative protein expression via Image Pro Plus software. GAPDH was used as an internal control. Data were presented as the mean ± standard error of the mean (n = 3). Statistically significant compared to the LV-NC group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The gels in d were cropped, full-length gels are presented in Additional file 1 Original Band of Western blot Analysis Fig. S3. The bands of BACH1, DSPP, HO-1 and GAPDH were from the same gel, while the bands of RUNX2 and DMP1 were from different gels