Fig. 3
The inhibition assay for binding of live Pg cells to sHAPs incubated with SIgA Ab-enriched saliva from mice administered nasally stat23 with/without dDA (A) or to pHAPs with SIgA Ab-enriched saliva from mice administered nasally prp21 with/without dDA (B). SIgA Ab-enriched saliva (100 µL) (i.e., saliva from which IgM and IgG Abs were removed using affinity columns chromatography) was added to sHAPs (A) or pHAPs (B) in siliconized glass tubes; the resulting mixtures were incubated for 3 h at room temperature. The HAPs were washed three times with 500 µL of KCl buffer. Subsequently, Pg ATCC 33,277 (1 × 108 cells in 200 µL of KCl buffer) was added to each tube. After incubation for another 3 h at room temperature, the HAPs were washed three times with 500 µL of KCl buffer and then transferred to white 96-well plates. The levels of ATP (indicative of live Pg cells bound to the HAPs) were determined using a luciferase assay and luminometer. Values are represented as the means ± SE from three replicates. Comparisons were performed by two-tailed unpaired Student’s t-test. **p < 0.01