Table 1 The primer sequences used in the real-time PCR.
Primer | Sequence (5’-3’) | |
|---|---|---|
hsa-miR-25-3p | RT primer | 5’-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGtcagaccg-3’ |
PCR primer (forward) | 5’-GCCGAGCAUUGCACUUGUCU-3’ | |
U6 | PCR primer (forward) | 5’-CCTCGCTTCGGCAGCACATATAC-3’ |
Universal reverse primer | Provided by Applied Biosystems | |