The prevalence of dental caries and associated factors among secondary school children in rural highland Vietnam

Background To determine the prevalence of dental caries in primary and permanent teeth and identify factors associated with dental caries among secondary school children in rural highland Vietnam. Methods This was a cross-sectional study that included 1985 secondary schoolchildren. Dental examination was performed at school using World Health Organization criteria. Data collection on demographic characteristics and knowledge, attitude, and practices related to dental caries was conducted by interviewing children. Descriptive and inferential statistics using a multivariate logistic regression model were applied. Results Prevalence of caries in primary and permanent teeth was 41.1 and 68.9 %, respectively. Prevalence of caries in primary teeth in the age group 11–12 years old (59.4 %) was significantly higher than in children in the age group of 13–14 years (27.8 %; p < 0.01). Factors associated with dental caries in primary teeth were age group of 11–12 years, belonging to the Jarai ethnic group, and having inadequate knowledge or attitude related to dental caries. Factors associated with dental caries in permanent teeth were having insufficient knowledge, attitude, and practices related to dental caries. Conclusions The prevalence of dental caries in primary and permanent teeth was high among secondary school children in Vietnam’s rural highlands. It is recommended that interventions focus on younger secondary school children and the Jarai minority ethnic group, and that interventions should emphasize improving knowledge, attitudes, and practices related to dental caries.

Vietnam has made remarkable progress in improving public health over the past few decades. However, an effective oral care strategy is still undeveloped and existing strategies have not met the needs of the population [14]. Although previous studies in Vietnam indicated a high prevalence of dental caries in primary and permanent teeth in children [14][15][16][17], these studies used outdated datasets or small sample sizes, or focused only on urban areas. To date, no studies regarding the prevalence of dental caries in children in rural highland areas of Vietnam have been conducted. Similarly, few studies have been conducted that investigated the factors associated with dental caries that include patient knowledge, attitudes, and practices related to dental caries. Thus, this study aims to determine the prevalence of dental caries in primary and permanent teeth and identify factors associated with dental caries in secondary school children in rural highland Vietnam.

Study design and setting
This was a cross-sectional study conducted from August through October 2017 in Gia Lai province, Vietnam. Gia Lai is located in the central highland region and is the second-largest province of Vietnam by area, and the majority of its population lives in rural and mountainous areas. In 2019, Gia Lai had a population of approximately 1.5 million people. In Vietnam, the Kinh is a majority ethnic group, whereas the Jarai is a minority ethnic group. This study was conducted in two rural districts in Gia Lai that are mainly inhabited by the Kinh and Jarai ethnic groups. The two rural districts were randomly selected among 14 rural districts of Gia Lai province. Study participants included secondary school students in the two districts. Approval to conduct the study was obtained from the Institutional Review Board of the Vietnam Military Medical University (decision no. 350/2016/YTCC-HD3, dated 29/12/2016). All procedures performed in the present study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the declaration of Helsinki 1975, which was revised in 2000.

Sample size and sampling
Inclusion criteria were children studying at secondary schools who obtained permission from their parents or guardians to participate in the study. We excluded children who had any chronic debilitating disease or who had a fever on the survey day. We calculated the sample size according to the formula for estimating a population proportion with specified absolute precision suggested by the WHO [18], and included an anticipated proportion of dental caries among children of 70 % based on a pilot study of 50 children in a single secondary school. The school surveyed in the pilot study was in the study area but was not included in this study. We estimated that the minimum sample size needed for this study was 323 children based on a significance level of 0.05 and an absolute precision of 0.1. Since secondary schools in Vietnam include four grades (grades six through nine), we multiplied our sample size by four to generate a total target sample size of 1292 children. Allowing for a nonresponse rate of 30 %, we estimated that we would need at least 1,845 children for this study. We invited 2057 children from six randomly-selected secondary schools from 24 secondary schools in two rural highland districts of Vietnam. The participation rate was 96.5 % (n = 1985), with a small number of children not participating due to non-consent by the child or their parents or non-attendance at school (n = 72).

Variables
We considered the presence of dental caries in primary or permanent teeth to be an outcome variable in this study. To identify caries, we performed a dental examination of children at the selected schools using the WHO dental caries diagnosis guidelines to identify the number of decayed teeth, teeth missing due to caries, and filled teeth, for both primary teeth and permanent teeth (dmft/ DMFT) [19]. We then calculated dmft and DMFT indices for primary and permanent teeth, respectively, to describe the status of children's dental caries, with dental caries in primary and permanent teeth defined as dmft > 0 and DMFT > 0, respectively.
We collected independent variable data including sex (girl or boy), age group (11-12 years old or 13-14 years old), ethnicity (Kinh or Jarai), and father's and mother's occupations (official or worker, farmer, or freelancer). We also collected data regarding study participants' knowledge, attitude, and practices related to dental caries through interviews. Interviews consisted of ten questions for each group of topics about knowledge, attitude, and practices related to dental caries for a total of 30 questions. Correct answers garnered one point, and incorrect answers garnered zero points. Scores (out of 10) for knowledge, attitudes and behaviors were summed for each child. Knowledge, attitudes and behaviors were rated as "good" if they scored 8 or more. Independent variables for multivariate analysis were good knowledge (yes or no), good attitude (yes or no), and good practices (yes or no).

Statistical analysis
We present quantitative variables as means and standard deviations and qualitative variables as frequencies and percentages. Differences between groups were analyzed according to Chi-square tests (qualitative variables). We used multivariate logistic regression analysis to calculate adjusted odds ratios (AOR) and identify any factors associated with dental caries. We conducted all statistical analyses using STATA version 14.0 (Stata Corp, College Station, TX, USA), and differences were considered significant at p-values < 0.05.

Results
A total of 1,985 secondary school children aged 11 through 14 years (in grades six through nine) were enrolled in the study. Table 1 indicates the characteristics of participants in this study. The proportions of girls to boys and that of children aged 11-12 years or 13-14 years were slightly different. Similarly, more children from the Jarai minority ethnic group were included than were children from the Kinh majority ethnic group. Figure 1 illustrates the proportion of children with good knowledge, good attitude, and good practices related to dental caries. Table 2 shows the prevalence of dental caries among children according to various factors. The overall prevalence of caries in primary and permanent teeth was 41.1 and 68.9 %, respectively. There were no significant differences in the prevalence of caries in primary or permanent teeth between boys and girls or prevalence in permanent teeth between the age group of 11-12 years and 13-14  years (all p > 0.05). However, the prevalence in primary teeth in the age group of 11-12 years (59.4 %) was significantly higher than in children in the age group of 13-14 years (27.8 %; p < 0.01). There were significant differences in the prevalence of caries in primary and permanent teeth between children who did and did not have good knowledge, attitude, and practices related to dental caries (all p < 0.01). Table 3 indicates results of the multivariate logistic regression analysis. Factors that were determined to be significantly associated with dental caries in primary teeth were age group of 11-12 years (AOR = 6.2; 95 % confidence interval [CI] = 4.9-7.9), belonging to the Jarai ethnic group (AOR = 1.4; 95 % CI = 1.2-1.8), and children with insufficient knowledge (AOR = 3.4; 95 % CI = 2.5-4.6) and attitude (AOR = 5.6; 95 % CI = 3.2-9.7) related to dental caries. Factors that were significantly associated with dental caries in permanent teeth were children with insufficient knowledge (AOR = 13.5; 95 % CI = 9.5-19.2), attitude (AOR = 14.1; 95 % CI = 8.3-23.9), and practices (AOR = 2.6; 95 %CI = 1.5-4.4) related to dental caries.

Discussion
This is the first study to report the prevalence of dental caries and associated factors in Vietnam's rural highlands, which face many difficulties compared to other regions in Vietnam. We found that the prevalence of dental caries was relatively high, especially in permanent teeth. We also found a difference in the prevalence of dental caries in primary teeth between children of the Jarai and Kinh ethnic groups. Furthermore, children with insufficient knowledge, attitudes, and practices related to dental caries were more likely to have dental caries than those who had good knowledge, attitudes, and practice. This study provides evidence related to dental caries so that policymakers can develop appropriate intervention plans for rural highland areas in Vietnam.
Dental caries is a concerning issue because it is common among children and negatively impacts a child's quality of life. Caries is a global public health challenge and is continuously studied and documented in various countries. In 2020, the global prevalence of dental caries in primary and permanent teeth was estimated at 46.2 and 53.8 %, respectively, which was considered to be high [20]. Since our study only included children aged 11-14 years, we report a prevalence of dental caries that varies slightly from those results. We do report findings that are consistent with other previous studies, which also indicated a high prevalence of dental caries among children [21][22][23][24][25]. The prevalence of dental caries in our study was higher than that reported by certain previous studies [21,22,24,26], but lower than that reported by other studies in the same age group [23,25]. This variation in reported prevalence of dental caries could be due to differences in economic conditions or strategies of preventive medicine in response to oral health problems in each country. In addition, differences may be due to differing sample sizes, sample selection methods, and study dates. However, the prevalence of dental caries among children was generally high in previous studies. The prevalence of dental caries tends to be lower in developed countries and higher in developing countries [20,27], suggesting that concrete and creative strategies still need to be developed to deal with dental caries, especially in developing countries. Furthermore, as dental caries may disproportionally affect disadvantaged groups [28,29], it is necessary to develop appropriate oral care public health policies that target marginalized socioeconomic groups.
The relatively high prevalence of dental caries that we found is consistent with previous studies conducted in  21:349 Vietnam [14,15,17], although it is slightly lower than the prevalence reported by Do et al., who began collecting data in 1999 [14]. However, when comparing by age group, our findings were quite similar to theirs. This is a considerable issue and suggests that, although there have been many positive socioeconomic changes in Vietnam in the 20 years between that study and this one, existing policies may not have been sufficient to improve public dental health in rural highland areas. Similarly, we found a lower prevalence of dental caries than did two other reports, although those studies focused on children in kindergarten and primary school age groups, who are at increased risk for dental caries [16,17]. We suggest that, given the number of studies focusing on younger children, future studies should also focus on children of secondary school age.
We also found a significant relationship between dental caries and knowledge, attitudes, and practices related to dental caries. Our results are similar to previous studies that also reported that children who recognized a dental health problem were less likely to develop dental caries [30,31]. However, like previous studies, we also found that the prevalence of good knowledge, attitude, and practices related to dental caries was low among schoolchildren [17,32,33]. In Vietnam, the School Oral Health Promotion Program has been implemented to improve students' understanding of oral health since 1980. However, Thuy et al. reported concern that the program has not improved oral health behavior among schoolchildren [17], indicating that policymakers should provide more effective plans to enhance oral health behavior among schoolchildren. In addition, water fluoridation rates in communities and schools have remained low, something that, if improved, could reduce the prevalence of dental caries among schoolchildren. Moreover, a relationship between parental behavior and dental caries among schoolchildren has been reported [34], suggesting that there is also a need for oral health education programs that target parents.
Whereas some previous studies revealed differences in the prevalence of dental caries between boys and girls [14][15][16], we found no differences, as did a study in India [7]. In addition, we observed that the prevalence of dental caries in the Jarai minority ethnic group was slightly higher than that in the Kinh majority ethnic group, although this difference was not statistically significant. In Vietnam, minority ethnic groups are considered to be vulnerable groups, so they often enjoy advantageous social benefits and government health policies, something that may have contributed to a narrowing of the disparity in disease prevalence between the Jarai and the Kinh ethnic groups. Even so, there may be differences in oral disease patterns among minority ethnic groups due to differing economic conditions within households from various groups. Therefore, we suggest that further research is needed in order to better understand inequalities in dental caries among schoolchildren so that more relevant interventions and policies are created in the future [35,36].
In this study, we found no difference in the prevalence of dental caries in permanent teeth according to age group. This finding differs from previous studies that reported a higher prevalence of dental caries in permanent teeth in older age groups [37,38]. This difference may be due to the age groups in our study were older than the age groups in previous studies. However, we did observe that the prevalence of dental caries in primary teeth differed according to age group, indicating that younger children had a higher risk of dental caries than did older children. This was also reported in previous studies [14,37,38], along with the finding that younger schoolchildren were more likely to have insufficient oral hygiene practices and improper dietary habits, and thus they were more likely to have dental caries.
We also report that the prevalence of dental caries differed according to mothers' occupations, although we found no difference when analyzing using a logistic regression model, likely due to the fact that most children's parents were farmers or freelancers and the study having been conducted in rural areas. There is no specific guideline for classifying occupations in Vietnam, so that task was difficult to apply in practice. In addition, because people in rural areas often have many jobs that are usually short-term, parents with very diverse jobs might all have been classified as freelancers. With such occupation demographics, it is possible that parental employment influences children's dental health [34,39]. Similarly, it is possible that children from families whose parents work in more stable jobs (e.g., officials or workers) are more likely to receive adequate oral health care. We suggest that more studies are needed in the future to learn more specifically the role of parental occupation in children's dental health.
We also wish to address some of the limitations of this study. The diagnosis of dental caries based solely on dental examination without using radiography may not detect all cases of dental caries. Although experienced researchers designed the questionnaires about knowledge, attitude, and practices related to dental caries, some children might not have understood the questions, thus affecting our results. Beyond that, the chosen cutoff threshold for determining good and insufficient knowledge, attitudes, and practices may have influenced our results. Furthermore, while parental education may also relate to children's oral health behavior, given that we interviewed the study participants at school, we did not collect information regarding parental education. It could also be a limitation related to the age examination because some teeth might not fully erupt. Finally, this study is a cross-sectional study and our results should not be interpreted to indicate causality.

Conclusions
The prevalence of dental caries in primary and permanent teeth was high among secondary school children in Vietnam's rural highlands, and the majority of surveyed secondary school children suffered dental caries in permanent teeth. Dental caries in primary teeth was associated with belonging to the age group 11-12 years old, belonging to the Jarai ethnic minority group, and having insufficient knowledge and practices related to dental caries. Meanwhile, dental caries in permanent teeth was associated with having insufficient knowledge, attitude, and practices related to dental caries. Therefore, in addition to implementing interventions that focus on younger secondary school children and the Jarai minority ethnic group, future interventions aimed at improving knowledge, attitudes, and practices for secondary school children are essential.