Figure 2
From: Salivary pellicles on titanium and their effect on metabolic activity in Streptococcus oralis
Images showing metabolic activity of S. oralis in suspension and adhered to uncoated titanium surfaces. Bacteria suspended in PBS were allowed to form biofilms on titanium surfaces in a parallel plate flow-cell system for 2 and 24 hours. The metabolic activity of the cells was assessed using the Bac Light CTC Vitality Kit. For adhered cells, DAPI was used as a counterstain. Cells were viewed with CSLM and the proportion of metabolically active (red/pink) cells assessed by manually counting at least 1000 cells. The scale bars represent 10 μm and the inserts show cells in twofold enlargement.