Figure 4

2DE gels of whole saliva and the salivary pellicle desorbed from titanium surfaces. Pooled whole human saliva (A) and salivary pellicles desorbed from titanium surfaces using detergent (B) were subjected to isoelectric focussing on pH 4–7 IPG strips followed by SDS-PAGE on 14% gels. Gels were stained with Coomassie Blue (A) or silver (B). Spots of interest were picked from the Coomassie gel, identified using LC-MS/MS and spot identities transferred to the silver gel. An explanation of the labels is given in Table 2.