Figure 4
From: Enamel matrix derivative protein enhances production of matrixmetalloproteinase-2 by osteoblasts
Effects of MEK on degradation of gelatin in EMD protein stimulated MG-63 cells. Concentrated conditioned media prepared from unstimulated MG-63 cells (lane 1), EMD protein-stimulated MG-63 cells (5 μM) (lane 2), EMD protein-stimulated MG-63 cells in the presence of DEMSO (lane 3), EMD protein-stimulated MG-63 cells cultured in the presence of SB203580 (5 μM) (lane 4) were separated on 8% SDS-PAGE. Membranes were blotted with anti-MMP-2 antibody and visualized with Super Signal west pico chemiluminescent substrate. Molecular weight markers (kDa) are shown in the left column. The same concentrated conditioned media were separated and transferred to membranes. Peak heights for each density are depicted as percent of maximum value.