Fig. 3
From: Effects of missense mutations in sortase A gene on enzyme activity in Streptococcus mutans
SDS-PAGE analysis of SrtA enzyme expression. Detailed legend: Expression of non-purified and purified SrtA analysed by SDS-PAGE. Lane M: Protein marker. Lane 1: Supernatant from E. coli BL21 transfected with pET32a. Lanes 2, 4, 6: Supernatants from E. coli BL21 transfected with pET32a-srtA △N120(UA159) , pET32a-srtA △N120(T168G) and pET32a-srtA △N120(G470A) , respectively, before purification. Lanes 3, 5, 7: Supernatants from E. coli BL21 transfected with pET32a-srtA △N120(UA159) , pET32a-srtA △N120(T168G) and pET32a-srtA △N120(G470A) , respectively, after purification using Ni sepharose 6 Fast Flow columns. New bands appeared in the supernatants from E. coli BL21 containing pET32a-srtA △N120(UA159) , pET32a-srtA △N120(T168G) and pET32a-srtA △N120(G470A) compared with the supernatant from E. coli BL21 containing pET32a. The estimated molecular weight of SrtA△N40(UA159) and the mutant enzymes was approximately 42 kDa as indicated by SDS-PAGE