Fig. 4
From: Effects of missense mutations in sortase A gene on enzyme activity in Streptococcus mutans
Western blot of SrtA△N40(UA159), SrtA△N40(D56E) and SrtA△N40(R157H) probed with rabbit anti-His tag monoclonal antibody. Detailed legend: Lane M: Marker. Lane 1: Supernatant from E. coli BL21 transfected with pET32a. Lanes 2, 3, 4: Supernatants from E. coli BL21 transfected with pET32a-srtA △N120(UA159) , pET32a-srtA △N120(T168G) and pET32a-srtA △N120(G470A) , respectively