Table 1 Comparison of the enzymatic activities of SrtA△N40(UA159) and the mutant enzymes
From: Effects of missense mutations in sortase A gene on enzyme activity in Streptococcus mutans
Enzyme | V max (μM · s-1) (×10-3) | k cat (s-1) (×10-3) | K m (μM) | k cat /K m (μM-1 · s-1) (×10-4) |
|---|---|---|---|---|
SrtA△N40(UA159) | 3.75 ± 0.15 | 3.04 ± 0.12 | 15.50 ± 0.25 | 1.96 ± 0.08 |
SrtA△N40(D56E) | 0.91 ± 0.13 | 0.74 ± 0. 11 | 15.34 ± 0.32 | 0.48 ± 0.06 |
SrtA△N40(R157H) | 2.78 ± 0.26 | 2.25 ± 0.21 | 15.35 ± 0.07 | 1.47 ± 0.14 |