Table 3 Method of platelet-rich plasma preparation in all selected randomized controlled clinical trials

Gupta G et al. 2014 [34]

PRP + HA

HA

Not reported

Two (1200 r.p.m., 20 min & 2000 r.p.m., 15 min)

10 % CaCl₂ mixed with human thrombin

Not recorded

Okuda K et al. 2005 [11]

PRP + HA

HA

Heraeus Labofuge 300

Two (2400 r.p.m., 10 min & 3600 r.p.m., 16 min)

0.1 g of sodium alginate

Not recorded directly; reference was made to a previous study [48], Baseline: 257 × 10³/μL ± 46 × 10³/μL

Hanna R et al. 2004 [35]

PRP + BDX

BDX

SmartPReP

Two (2400 r.p.m., 10 min & 3600 r.p.m., 15 min)

1 mL of 10 % CaCl2, mixed with 1000 United States, Units of topical thrombin

Not recorded

Ouyang XY et al. 2006 [36]

PRP + ABB

ABB

Universal 16R centrifuge

Two (1220 r.p.m., 15 min & 3600 r.p.m., 15 min)

Sterile saline solution containing 10 % CaCl₂ mixed with 100 U/mL sterile bovine thrombin

Baseline: 189 × 10³/μL ± 37 × 10³/μL., Post treatment: 680× 10³/μL ± 103 × 10³/μL

Döri et al. 2009 [37]

PRP + ABB

ABB

Curasan PRP kit

Two (1220 r.p.m., 15 min & 3600 r.p.m., 15 min)

Sterile saline solution containing 10 % CaCl₂ mixed with 100 U/mL sterile bovine thrombin

Not recorded directly; reference was made to a previous study [49], Post treatment: 2519.6 × 10³/μL ± 834.3 × 10³/μL

Demir et al. 2007 [38]

PRP + BG

BG

Heraeus Christ Medifuge

Two (3000 r.p.m., 10 min & 3600 r.p.m., 10 min; or 200 g, 10 min

0.3 mL of 0.025 M CaCl_2, mixed with blood harvested from the surgical site

Baseline: 189× 10³/μL ± 37 × 10³/μL, Post treatment: 680 × 10³/μL ± 103× 10³/μL

Agarwal et al. 2014 [39], Piemontese et al. 2008 [40]

PRP + DFDBA

DFDBA

SmartPReP

Two (2400 r.p.m., 10 min & 3600 r.p.m., 15 min)

1 mL of 10 % CaCl₂ mixed, with 1000 United States, Units of topical thrombin

Not recorded

Harnack et al. 2009 [41]

PRP + β-TCP

β-TCP

Curasan PRP kit

Two (3169 r.p.m., 10 min & 4725 r.p.m., 15 min; or 900 g, 10 min & 2000 g, 15 min)

Blood harvested from the, surgical site

Not recorded

Özdemir et al. 2012 [42]

PRP + β-TCP

β-TCP

Curasan PRP kit

Two (2400 r.p.m., 10 min & 3600 r.p.m., 15 min)

Not recorded

Baseline: 290 × 10³/μL ± 86 × 10³/μL. Post treatment: 1075× 10³/μL ± 636 × 10³/mL

Kaushick et al. 2007 [43]

PRP + β-TCP + HA

β-TCP + HA

Not reported

Two (5000 r.p.m., 10 min & 2000 r.p.m., 10 min)

10 % CaCl₂ mixed with an equal volume of saline.

Baseline: 200 × 10³/μL Post treatment: 1250 × 10³/μL.

Christgau et al. 2006 [44]

PRP + β-TCP + GTR

β-TCP + GTR

Spectra cell separator

Not recorded

0.5 ml of a sterile 10 % CaCl₂ solution

Baseline: 273× 10³/μL ± 56 × 10³/μL, Post treatment: 2134 × 10³/μL ± 782× 10³/μL

Döri et al. 2007 [45], Döri et al. 2007 [46] Döri et al. 2008 [47]

PRP + ABB + GTR PRP + NBM + GTR PRP + β-TCP + GTR

ABB + GTR NBM + GTR β-TCP + GTR

Curasan PRP kit

Two (1220 r.p.m., 15 min & 3600 r.p.m., 15 min)

Sterile saline solution containing 10 % CaCl₂ mixed with 100 U/mL sterile bovine thrombin

Not recorded directly; reference was made to a previous study [49], Post treatment: 2519.6 × 10³/μL ± 834.3 × 10³/μL