Fig. 4

ZDHHC16 suppressed p-CREB expression

ZDHHC16 protein expression was promoted, and p-CREB protein was inhibited by ZDHHC16 plasmid of the DPSCs (A1, A2, A3); ZDHHC16 protein expression was inhibited, and p-CREB protein was promoted by Si-ZDHHC16 plasmid of the DPSCs (B1, B2, B3); ZDHHC16 expression was induced, and p-CREB expression was suppressed by ZDHHC16 plasmid of DPSCs (×200, Immunofluorescence, C), ZDHHC16 protein interlinked CREB protein (D), CREB agonist induced p-CREB and GPX4 protein expressions of DPSCs by ZDHHC16 up-regulation (E1, E2,E3); CREB inhibitor suppressed p-CREB and GPX4 proteins of DPSCs by down-regulation of ZDHHC16 (F1, F2, F3).

**p < 0.01 compared with negative or si-nc, ##p < 0.01 compared with ZDHHC16 or si-ZDHHC16.