Figure 4

Study procedure and experimental set-up. a: - Specimen preparation from permanent bovine incisors - Enamel and dentin surfaces ground flat and polished. b: - Artificial lesion creation in half of the enamel specimens. c: - Sterilization (ethylene oxide) of all specimens (enamel and dentin). d: - Insertion of four specimens into removable dentures during each phase (2 enamel specimens and 2 dentin specimens) - Blinded medication and fluoride-free toothpaste. e: - Preparation of thin sections (100 μm). f: - Microradiographic analysis of thin sections and statistical analysis