Figure 5
From: Enamel matrix derivative protein enhances production of matrixmetalloproteinase-2 by osteoblasts
p38 MAPK pathways are important for EMD protein-stimulated MG-63 cell-mediated degradation of gelatin. Glass was coated with 100 μg/ml of quenched fluorescent substrate DQ-gelatin. MG-63 cells were treated for 30 min with U0126 (5 μM). Cells were incubated in the presence or absence of 100 μg/ml EMD protein for 20 h. Degradation of gelatin (green fluorescence) was detected using a confocal microscope (Excitation: 488 nm; Emission: 530 nm). Images were obtained at ×40 magnification (A through I). Differential interference contrast (DIC) images are shown. Density is depicted as mean GFP per cell.