Figure 3
From: Role of extracytoplasmic function sigma factors in biofilm formation of Porphyromonas gingivalis
Biofilm formation by homotypicP. gingivalis33277, ECF sigma factor mutant and complemented mutant strain. The strains were grown on enriched BHI broth anaerobically at 37°C in collagen type-I-coated 12-well flat-bottom microplates. After 48 h of cultivation, the organized biofilm mass was evaluated by staining with crystal violet. (a) Biofilm formation of 33277, PGN_0274 mutant and the complemented mutant strain were compared. (b) Biofilm formation of 33277, PGN_1740 mutant and the complemented mutant strain were compared. Biofilm formation determined by crystal violet staining and adjusted for growth (A600 units per OD660 unit). The data shown are mean ± SD of triplicate experiments. *, p < 0.05, and ***, p < 0.001, by a one-way ANOVA Test/Dunnett’s Multiple Comparison Test.