Table 1 Oligonucleotide PCR primers utilized
Name | Sequence | Anneal. Temp. | |
|---|---|---|---|
DNA Sequencing | |||
HBD1 SEQ | AACTCTAGCAGGTACCAGAGCTTACCT | 65 | |
CTAACCTAGAAAACCAAACAGGAGGAG | |||
DEFB1 SNEST | TGAGGCCATCTCAGACAAAA | 65 | |
GCTCCAGGCGTAAAGCTAAA | |||
QPCR | Primer Efficiency | ||
HBD1 | CACTTGGCCTTCCCTCTGTA | 1.91 | 56 |
CGCCATGAGAACTTCCTACC | |||
HBD2 | GGAGCCCTTTCTGAATCCGCA | 1.98 | 65 |
CCAGCCATCAGCCATGAGGGT | |||
HBD3 | GTGAAGCCTAGCAGCTATGAGGAT | 1.68 | 60 |
TGATTCCTCCATGACCTGGAA | |||
RPO | GCCTTGACCTTTTCAGCAAG | 2.04 | 59 |
GCAGCATCTACAACCCTGAAG | |||
Beta-actin | CTCTTCCAGCCTTCCTTCCT | 1.75 | 63 |
AGCACTGTGTTGGCGTACAG | |||