Fig. 2

The protease activities of P. gingivalis supernatant or TLCK-preincubated supernatant. Protease activity was measured with a Protease Assay™ Kit and was monitored for 4, 8 and 16 h. The absorbance of the dye-labeled peptide was measured at 570 nm for determination of the protease activity. Native supernatant showed significantly higher proteolytic activity than TLCK-preincubated supernatant after 4 and 8 h. Triplicate measurements were performed. Data are shown as the mean with the standard deviation (mean ± SD). *, P < 0.05, compared with native supernatant values