In situ remineralisation response of different artificial caries-like enamel lesions to home-care and professional fluoride treatments

Table 1 Description of the demineralising protocols applied in vitro

Demineralisation*		pH	Period
MC Gel	8 % methylcellulose gel layer of 0.5 cm at 4 °C. After 12 h, covered with an equal volume (1.5 mL) of 0.1 M lactic acid.	pH 4.6	14 days
PA Gel	20 g/L Carbopol 907 (PA, molecular weight 450,000 Da), 500 mg/L hydroxyapatite and 0.1 M lactic acid.	pH 4.8	16 h
PA Gel	25 mL gel/ specimen	pH 4.8	16 h
TEMDP Solution	3 mM CaCl₂ · 2H₂O (LabSynth), 3 mM KH₂PO₄ (Sigma-Aldrich), 50 mM lactic acid (Sigma-Aldrich), 6 μM TEMDP (Sigma-Aldrich); traces of thymol.	pH 5.0	6 days
TEMDP Solution	30 mL solution/ specimen.	pH 5.0	6 days
Buffer Solution	1.28 mM Ca(NO₃)₂ · 4H₂O (Merck), 0.74 mM NaH₂PO₄ · 2H₂O (LabSynth), 50 mM glacial acetic acid and 0.03 ppm F (NaF).	pH 5.0	16 h
Buffer Solution	30 mL solution/specimen	pH 5.0	16 h

ISSN: 1472-6831