Fig. 2
From: Molecular and clinical analyses of Helicobacter pylori colonization in inflamed dental pulp
Sensitivity of the single and nested PCR assays for H. pylori detection. Representative images showing the sensitivities of (a) single and (b) second step PCR assays for the detection of H. pylori J99 genomic DNA. Representative images showing the sensitivities of (c) single and (d) second step PCR assays for the detection of H. pylori bacterial DNA from inflamed pulp. Representative images showing the sensitivities of (e) single and (f) second step PCR assays for the detection of H. pylori bacterial DNA added to non-infected dental pulp. The ureA-aF and ureA-bR primer sets were used for the single PCR assay and ureA-bF and ureA-aR primer sets were used for the second step PCR assay. M, molecular size marker (100 bp DNA ladder)