Table 1 Primer sequences
From: The role of osteomodulin on osteo/odontogenic differentiation in human dental pulp stem cells
Gene | Primer sequences |
|---|---|
For lentiviral constructs | |
 shOMD_F | 5’-CCGGGATCACGATGATCCTGACAATCTCG -AGATTGTCAGGATCATCGTGATCTTTTTG-3’ |
 shOMD_R | 5’-AATTCAAAAAGATCACGATGATCCTGACA -ATCTCGAGATTGTCAGGATCATCGTGATC-3’ |
 non-target shRNA_F | 5’-CCGGCAACAAGATGAAGAGCACCAACTC -GAGTTGGTGCTCTTCATCTTGTTGTTTTTG-3’ |
 non-target shRNA_R | 5’-AATTCAAAAACAACAAGATGAAGAGCAC CAACTCGAGTTGGTGCTCTTCATCTTGTTG-3’ |
For real-time qPCR and RT-PCR | |
 OMD_F | 5′- AGGCTGTGTCAGTGAATGCT-3’ |
 OMD_R | 5′- GTTGCTGAATGTGCATCGGA-3’ |
 DSPP_F | 5’-GCCATTCCAGTTCCTCAAAGC-3’ |
 DSPP_R | 5’-CATGCACCAGGACACCACTT-3’ |
 DMP 1_F | 5’-ATGCCTATCACAACAAACC-3’ |
 DMP 1_R | 5’-CTCCTTTATGTGACAACTGC-3’ |
 ALP_F | 5’-GGACCATTCCCACGTCTTCAC-3’ |
 ALP_R | 5’-CCTTGTAGCCAGGCCCATTG-3’ |
 β-actin_F | 5’-TGGCACCCAGCACAATGAA-3’ |
 β-actin_R | 5’-CTAAGTCATAGTCCGCCTAGAAGCA-3’ |