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Table 1 Enrollment criteria

From: Comparing dental plaque microbiome diversity of extrinsic black stain in the primary dentition using Illumina MiSeq sequencing technique

Inclusion criteriaExclusion criteria
EBS-positive subjects
 Having at least 10 teeth with EBS in the oral cavity, with score 2–3Receiving antibiotics within 3 months before the study
 3–5 years of age (primary dentition)Receiving any kind of polishing within 3 months before the study
 Free of systemic diseasesHaving intrinsic black stain
 Free of caries, gingivitis or periodontitis, etc. 
 Written informed consent 
EBS-free subjects
 Age, gender and dentition status comparable with EBS-positive patientsReceiving antibiotics within 3 months before the study
 Free of any kind of teeth extrinsic stainReceiving any kind of polishing within 3 months before the study
 Free of systemic diseasesHaving intrinsic black stain
 Free of caries, gingivitis or periodontitis, etc. 
 Written informed consent