Inclusion criteria | Exclusion criteria |
---|---|
EBS-positive subjects | |
Having at least 10 teeth with EBS in the oral cavity, with score 2–3 | Receiving antibiotics within 3 months before the study |
3–5 years of age (primary dentition) | Receiving any kind of polishing within 3 months before the study |
Free of systemic diseases | Having intrinsic black stain |
Free of caries, gingivitis or periodontitis, etc. | |
Written informed consent | |
EBS-free subjects | |
Age, gender and dentition status comparable with EBS-positive patients | Receiving antibiotics within 3 months before the study |
Free of any kind of teeth extrinsic stain | Receiving any kind of polishing within 3 months before the study |
Free of systemic diseases | Having intrinsic black stain |
Free of caries, gingivitis or periodontitis, etc. | |
Written informed consent |