Fig. 1

Schematic of the rotating-disc biofilm reactor system used to grow the Streptococcus mutans biofilms. The rotating-disc biofilm reactor is shown in the continuous flow mode of operation. The reactor was used to develop a standardized three-day S. mutans (ATCC 25175) biofilm on resin composite discs (Premise Flowable, Kerr). The size of the resin composite disc was standardized to 6 mm in diameter and 1.5 mm in thickness. The rotor was composed of a magnetic stir bar, on which a rotating-disc was attached to hold resin composite discs. The container equipped with the stirring bar was placed on a magnetic stirrer and incubated while rotating. Disc rotation provided continuous mixing of the solution added to the system