Fig. 1

Representative confocal images of E. faecalis biofilms of different groups stained using live/dead bac light bacterial viability kit; (A, B) control after 3 days; (C) control after 7 days; (D) 2%CHX after 7 days; (E) 41% Ca(OH)2 after 7 days; (F) 2%k21 after 3 days; and (G) 2%k21 after 7 days. Excitation was performed at λ = 514 nm. Green indicates a high level of bacterial viability in control specimens. Most of the red fluorescence in 2%k21 specimens indicating dead cell population. Scale bar = 50 μm