Fig. 3

CLSM images of SYTO 9/PI-stained biofilms. Biofilms formed in the absence (a–c) or presence of 5 mg/ml GC (d–f) or GCP (g–i) for 24 h were washed in PBS and stained with SYTO 9 (green fluorescence) and PI (red fluorescence). The images are three-dimensional reconstructions of all layers captured using the NIS Element software. a, d, g are merged images of SYTO 9/PI. b, e, h are SYTO 9 staining. c, f, i are PI staining