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Fig. 2 | BMC Oral Health

Fig. 2

From: Plant-based oral care product exhibits antibacterial effects on different stages of oral multispecies biofilm development in vitro

Fig. 2

Antibacterial effect of REPHA-OS® on biofilm formation. a Tukey Box Plots of biofilm volume, b mean value ± standard deviation of the live/dead distribution and c Tukey Box Plots of metabolic activity determined by resazurin assay of bacteria in biofilm after 24 h incubation with REPHA-OS® or the corresponding ethanol concentration. Purple brackets show significant differences to the BHI/VitK red control and black brackets show significant differences between REPHA-OS® and the corresponding ethanol concentration, each at a significance level of p ≤ 0.05. d—top Control biofilm (BHI/VitK) and d—bottom biofilm incubated with 5% REPHA-OS® after 24-h growth, live/dead fluorescence staining and subsequent confocal laser scanning microscopy. Viable bacteria are shown in green, dead bacteria in red/yellow. The 3D reconstruction was created with the software Bitplane Imaris. Scale bar = 50 µm

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