Fig. 3
Effect of BAFF inhibition on alveolar bone loss and osteoclast formation. a Representative images of alveolar bone resorption from ligature mice model. P, palatal surface. b, c Representative images of HE staining of the periodontal tissue from ligature mice model. Blue arrows, cemento-enamel junction (CEJ); yellow arrows, junctional epithelium (JE). Final magnification 200×, 400×. d, e Representative images of TRAP staining from ligature mice model. Arrows indicate TRAP-positive osteoclasts. R, root; AB, alveolar bone. Final magnification 100×, 200×. f Bone resorption area was calculated from cemento-enamel junction to the alveolar bone crest of the second maxillary molar at palatal groove site. g The percent of inflammatory cell infiltration in the periodontal tissues of mice was countered after HE staining. h Comparison of osteoclasts number/500 × 500 µm2 of bone surface area among groups. Data are presented as mean ± standard deviation. ***P < 0.001 using one-way ANOVA with Tukey's test. ANOVA analysis of variance, TRAP tartrate-resistant acidic phosphatase