Fig. 3

miR-30a-5p induced inflammation and inhibited osteogenesis. A The expression of miR-30a-5p was examined by qPCR in MC3T3-E1 cells after 24 h after transfected with relative controls (NC), miR-30a-5p mimics (mimic) and control. B The expression of miR-30a-5p was examined by qPCR in MC3T3-E1 cells after 24 h after transfected with relative controls (NC), miR-30a-5p inhibitors (inhibitors) and control. C The expression of IL1-R1, IL1-R2 and TNFR1 were examined by qPCR in MC3T3-E1 cells after 24 h after transfected with relative controls (NC), miR-30a-5p mimics (mimic) and control. D The expression of IL1-R1, IL1-R2 and TNFR1 were examined by qPCR in MC3T3-E1 cells after 24 h after transfected with relative controls (NC), miR-30a-5p inhibitor and control. E The level of ALP was examined by ALP staining in MC3T3-E1 cells after 7 days after transfected with miR-30a-5p mimics, miR-30a-5p inhibitors and relative controls (control). F The level of Alizarin red was examined by ALP staining in MC3T3-E1 cells after 21 days after transfected with miR-30a-5p mimics, miR-30a-5p inhibitors and relative controls (control). G The expression of ALP, Runx2, OC and Col1 were examined by qPCR in MC3T3-E1 cells after 24 h after transfected with relative controls (NC), miR-30a-5p inhibitor and control. H The expression of ALP, Runx2, OC and Col1 were examined by qPCR in MC3T3-E1 cells after 24 h after transfected with relative controls (NC), miR-30a-5p mimics and control. All data are shown as mean ± SEM. *p < 0.05; **p < 0.01