Comparative evaluation of the effect of cold ceramic and MTA-Angelus on cell viability, attachment and differentiation of dental pulp stem cells and periodontal ligament fibroblasts: an in vitro study

Table 1 Primer sequences (Seq. (5–3) of the target differentiation markers

Number	Name	Seq. (5–3)	TM	Per 13–20 µl (total volume) (µl)	Concentration (p/mol)
1	H-beta Actin-F	CTT CCT TCC TGG GCA T	60	1	10
	H-beta Actin-R	GTC TTT GCG GAT GTC CA
2	DSPP-F	CCA TTC CAG TTC CTC AAA G	60	1	10
	DSPP-R	TGG CAT TTA ACT CAT CCT GTA
3	ALP-F	GCA CCT GCC TTA CTA ACT	60	1	10
	ALP–R	AGA CAC CCA TCC CAT CT
4	DMP F	TTCTTTGTGAACTACGGAG	60	1	10
	DMP R	TTGATACCTGGTTACTGGG
5	H-collagen1α1–F	GCC AAG GGT CTG ACT G	60	1	10
	H-collagen1α1–R	CCC ATC ACA CCA GCC T

Beta Actin was used as the housekeeping-gene. F, Forward; R, Reverse; concentration used = 10 p/mol, volume = 1 µl, melting temperature (Temperature melting (TM) = 60 °C

ISSN: 1472-6831