Table 2 Characterization of the DNA inserts from the resistant clones found in the oral metagenomic library
Sample name (Accession number) | Size (bp) | BlastN | BlastX | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Closest homologue | Percentage identity (%) | Coverage (%) | Accession number of the homologous DNA (BlastN) | Closest homologue | ORF size (bp) | Percentage identity (%) | Coverage (%) | Position on sample | Accession number of the homologous proteins (BlastX) | ||
Sodium hypochlorite- 1 (MZ955857) | 12,588 | Rothia mucilaginosa strain FDAARGOS_369 chromosome, complete genome | 90.32 | 77 | CP023510.1 | Helix-turn-helix transcriptional regulator [Rothia mucilaginosa] | 168 | 100 | 41 | 2 → 169 | WP_049346502.1 |
MULTISPECIES: DUF3046 domain-containing protein [Rothia] | 222 | 100 | 100 | 347 → 568 | WP_049333114.1 | ||||||
MULTISPECIES: Recominase RecA [Rothia] | 1107 | 99 | 100 | 979 → 2085 | WP_049332756.1 | ||||||
RecX family trancriptional regulator [Rothia sp. HMSC072E10] | 1938 | 97 | 100 | 2292 → 4229 | WP_070680682.1 | ||||||
Hemin ABC transporter substrate-binding protein [Rothia mucilaginosa] | 1476 | 98 | 100 | 4391 ← 5866 | WP_152901671.1 | ||||||
MULTISPECIES: tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB [Rothia] | 1515 | 100 | 100 | 5883 → 7397 | WP_049332750.1 | ||||||
tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA [Rothia mucilaginosa] | 930 | 99 | 100 | 7474 → 8403 | WP_193389320.1 | ||||||
MULTISPECIES: Diaminopimelate epimerase [Rothia] | 936 | 100 | 100 | 8501 → 9436 | WP_049344416.1 | ||||||
MULTISPECIES: Methyltransferase [Rothia] | 624 | 100 | 100 | 9603 ← 10,226 | WP_049332745.1 | ||||||
GTPase HflX (Rothia sp. HMSC068F09) | 1581 | 99 | 94.3 | 10,426 → 12,006 | WP_070648960.1 | ||||||
ATP-dependent DNA helicase [Rothia sp. HMSC072B03] | 472 | 96 | 22.6 | 12,117 → 12,588 | WP_070482790.1 | ||||||
Chlorhexidine- 1 (MZ955858) | 1015 | No significant similarity found | – | – | – | Type IV secretion system DNA-binding domain-containing protein [Kocuria indica] | 237 | 51 | 10.8 | 746 → 982 | WP_121544318.1 |
Chlorhexidine- 2 (MZ955859) | 1536 | Streptococcus parasanguinis ATCC 15,912, complete genome | 99.28 | 92 | CP002843.1 | MULTISPECIES: Beta-ketoacyl-ACP synthase II [Streptococcus] | 659 | 100 | 53.2 | 1 → 659 | WP_014712692.1 |
MULTISPECIES: Acetyl-CoA carboxylase biotin carboxyl carrier protein [Streptococcus] | 489 | 100 | 100 | 663 → 1151 | WP_061604604.1 | ||||||
MULTISPECIES: 3-hydroxyacyl-ACP dehydratase FabZ [Bacteria] | 267 | 99 | 62.4 | 1270 → 1536 | WP_003004019.1 | ||||||
Erythromycin-1 (MZ955860) | 5528 | Streptococcus mitis strain SK637 chromosome, complete genome | 99.74 | 96 | CP028415.1 | Hypothetical protein [Streptococcus mitis] | 432 | 100 | 99.3 | 223 → 654 | CDG57854.1 |
MULTISPECIES: Hypothetical protein [Bacteria] | 336 | 97 | 100 | 651 → 986 | WP_000806926.1 | ||||||
YolD-like protein [Streptococcus pneumoniae] | 369 | 99 | 100 | 998 → 1366 | VLH51388.1 | ||||||
MULTISPECIES: Hypothetical protein [Bacteria] | 300 | 100 | 100 | 1354 → 1654 | WP_001072467.1 | ||||||
ABC-F type ribosomal protection protein Msr(D) [Streptococcus pneumoniae] | 2064 | 99 | 100 | 1771 ← 3234 | WP_000420316.1 | ||||||
Macrolide-efflux protein [Streptococcus pneumoniae] | 1194 | 98 | 93 | 3379 ← 4572 | VQI18798.1 | ||||||
Hypothetical protein [Streptococcus pneumoniae] | 186 | 100 | 100 | 4960 ← 5145 | AAR22392.1 | ||||||
Triclosan-1 (MZ95561) | 5408 | Streptococcus sp. oral taxon 431, complete genome | 91.83 | 99 | CP014264.1 | Enoyl-[acyl-carrier-protein] reductase FabK [Streptococcus sp. SK643] | 1020 | 96 | 100 | 629 → 1648 | WP_196793360.1 |
ACP S-malonyltransferase [Streptococcus sp. HMSC034E03] | 921 | 98 | 100 | 1641 → 2561 | WP_075232029.1 | ||||||
3-oxoacyl-[acyl-carrier-protein] reductase [Streptococcus sp. HMSC034E03] | 735 | 99 | 100 | 2595 → 3329 | WP_075232030.1 | ||||||
Beta-ketoacyl-ACP synthase II [Streptococcus mitis] | 1236 | 99 | 100 | 3352 → 4587 | WP_075232031.1 | ||||||
MULTISPECIES: 3-hydroxyacyl-ACP dehydratase FabZ [Streptococcus] | 384 | 100 | 91.4 | 5025 → 5408 | WP_042901453.1 | ||||||
Triclosan-2 (MZ955862) | 3944 | Streptococcus salivarius strain NCTC8618 genome assembly, chromosome: 1 | 99.75 | 97 | LR134274.1 | MULTISPECIES: Enoyl-CoA hydratase [Streptococcus] | 389 | 100 | 49 | 1 → 389 | WP_037601410.1 |
MULTISPECIES: Ketoacyl-ACP synthase III [Streptococcus] | 963 | 99 | 100 | 926 → 1888 | WP_037612035.1 | ||||||
MULTISPECIES: Enoyl-[acyl-carrier-protein] reductase FabK [Streptococcus] | 966 | 100 | 100 | 2283 → 3248 | WP_022495984.1 | ||||||
ACP S-malonyltransferase [Streptococcus salivarius] | 627 | 100 | 73.2 | 3318 → 3944 | WP_049545679.1 | ||||||
Tetracycline-1 (MZ955863) | 6366 | Streptococcus australis strain NCTC13166 genome assembly, chromosome: 1 | 93.08 | 99 | LS483444.1 | Tetracycline efflux ABC transporter Tet(46) subunit A [Streptococcus parasanguinis] | 1725 | 99 | 97.5 | 380 → 2104 | WP_125826751.1 |
Tetracycline efflux ABC transporter Tet(46) subunit B [Streptococcus parasanguinis] | 1737 | 99 | 100 | 2106 → 3842 | WP_049497012.1 | ||||||
MULTISPECIES: rRNA maturation RNase YbeY [Streptococcus] | 498 | 99 | 100 | 3939 → 4436 | WP_003005431.1 | ||||||
MULTISPECIES: GTPase Era [Streptococcus] | 900 | 99 | 100 | 4845 → 5744 | WP_003004993.1 | ||||||
MULTISPECIES: DNA-formamidopyrimidine glycosylase [Streptococcus] | 577 | 98 | 67 | 5790 → 6366 | WP_070587080.1 | ||||||