Fig. 2

The effect of HG on autophagy. a The relative LC3 II and P62 mRNA was measured by qRT-PCR. LC3 II was measured by LSCM (b) and western blotting (c) analysis. Scale bar = 15 μm. The ratio of LC3 II/LC3 I was assembled from the statistical analysis. Significant differences are shown as *P < 0.05 versus NG 12 h. ^#P < 0.05. The original picture of western blot of Fig. 2c was shown in Additional file 1: Fig. S2c