Fig. 3

The effect of high glucose HG on mitophagy. HGECs were challenged with or without 25 mmol/L glucose for 12 h and 48 h. a FUNDC1, NIX, and PINK1 mRNA levels were determined by qRT-PCR. b The fluorescence of PINK1 was detected by LSCM. c. The protein level of PINK1 was measured by western blotting (representative images and statistical analysis). Significant differences are shown as *P < 0.05 versus NG 12 h. ^#P < 0.05. The original picture of western blot of Fig. 3c was shown in Additional file 1: Fig. S3c