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Fig. 3 | BMC Oral Health

Fig. 3

From: Sodium butyrate inhibits osteogenesis in human periodontal ligament stem cells by suppressing smad1 expression

Fig. 3

Sodium butyrate (NaB) decreased the osteogenic capacity of periodontal ligament stem cells (PDLSCs). a Evaluation of alkaline phosphatase (ALP) activity 5 days after osteogenic induction indicated that 1 mM NaB decreased ALP activity in PDLSCs. b, c Alizarin red staining assay and cetylpyridinium chloride assay performed 14 days after induction showed that 1 mM NaB decreased the formation of mineralized nodules in PDLSCs. d–g Following 7 days of osteogenic induction, RT-PCR showed that 1 mM NaB downregulated Runx2, OSX, OCN, and OPN. h Western blotting showed that 1 mM NaB reduced the protein expression of Runx2, OSX, OCN, OPN, Smad1 and p-Smad1/5/8. i Following 14 days of osteogenic induction, RT-PCR showed that 0.1 mM and 1 mM NaB downregulated OCN. j Western blotting and densitometric analysis showed that 0.1 mM and 1 mM NaB reduced the protein expression of OCN. The length of the blots was appropriate in the manuscript. Error bars represent the standard deviation (n = 3). *p < 0.05, **p < 0.01, using one-way ANOVA

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BMC Oral Health

ISSN: 1472-6831

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