Fig. 6
From: Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis
Knockdown of LINC02147 promoted fibrogenesis in hBMFs. A The expression level of LINC02147 in the subcellular fractions of hBMFs was detected by qPCR. GAPDH and U6 were used as cytoplasmic and nuclear markers, respectively. B RNA FISH assay was used to determine the subcellular location of LINC02147 in hBMFs. Nuclei were stained with DAPI. Scale bar = 10 μm. C–G qPCR analysis of LINC02147, α-SMA, COL1α1, fibronectin (FN1), and vimentin in hBMFs with NC siRNA or LINC02147 siRNA. H–L Western blot results of α-SMA, COL1α1, FN1, and vimentin in hBMFs with NC siRNA or LINC02147 siRNA. The blots images were cropped by ImageJ (version1.8.0.112), and full-length blots are presented in Additional files 5 and 6: Figs. S2, S3. Unpaired t-test was used to compare gene expression between two groups (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)