Fig. 2
From: Oxidative stress impairs the calcification ability of human dental pulp cells
H2O2 attenuates ALP activity of hDPCs. A(a) A macroscopic view of wells showing hDPCs cultured in growth medium, hDPCs cultured in osteo-odontogenic medium, and hDPCs treated with 100 μM H2O2 in osteo-odontogenic medium. (b) A(a) panels were converted to RGB stack, and “blue slices” were picked up. (c) The thresholds of the images of A(b) were adjusted, and a limited threshold area is highlighted in red. B The stained area was quantified using Image J software on digitized photomicrographs (A(c)) captured by a Windows-based computer. Values are expressed as means ± SD of three independent experiments. **P < 0.01, Student’s t-test