Fig. 3.
From: Oxidative stress impairs the calcification ability of human dental pulp cells
2-APB reverses H2O2-decreased ALP mRNA expression and ALP activity of hDPCs. A ALP mRNA was determined by real-time PCR. Graphs show the ratio of ALP mRNA to GAPDH mRNA. Values represent means ± SD of three cultures. *p < 0.05, **p < 0.01: differs significantly from the control. B(a) A macroscopic view of wells showing hDPCs cultured in growth medium, hDPCs cultured in osteo-odontogenic medium, hDPCs treated with 100 μM H2O2 in osteo-odontogenic medium, and hDPCs treated with 10 μM 2-APB before the addition of 100 μM H2O2 in osteo-odontogenic medium. (b) B(a) panels have been converted to RGB stack, and “blue slice” images were picked up. (c) The thresholds of B(b) images were adjusted, and a limited threshold area is highlighted in red. C The stained area was quantified using the software NIH Image J® on digitized photomicrographs (B(c)) captured by a Windows-based computer. Data are shown as means ± SD of three independent experiments. *P < 0.05, **P < 0.01, one-way ANOVA with Tukey’s test