Fig. 4
From: Oxidative stress impairs the calcification ability of human dental pulp cells
Assessment of hDPC calcification with Alizarin red staining. A Panels show hDPCs cultured in growth medium (a), hDPCs cultured in osteo-odontogenic medium (b), hDPCs treated with 100 μM H2O2 in osteo-odontogenic medium (c) and hDPCs treated with 10 μM 2-APB before the addition of 100 μM H2O2 in osteo-odontogenic medium (d). Bars: 100 μm. B The amount of calcium was quantified using Image J software on digitized photomicrographs captured by a Windows-based computer. Data are presented as means ± SD of three independent experiments. **P < 0.01, one-way ANOVA with Tukey’s test