Fig. 5.
From: Oxidative stress impairs the calcification ability of human dental pulp cells
2-APB inhibits the ROS production induced by H2O2 in hDPCs. A ROS production in hDPCs. Fluorescence signals were detected with a confocal microscope. (a) control, (b) H2O2 100 μM, (c) H2O2 100 μM + 2-APB 10 μM, (d) 2-APB 10 μM. Bars: 100 μm. B The graph shows quantitative results of ROS production from three independent experiments. Per well, three pictures were taken at random. FITC fluorescence intensity from an area of 2 mm square from each picture was analyzed using Image J software on digitized photomicrographs captured by a Windows-based computer. Data represent means ± SD. *P < 0.05, **P < 0.01, one-way ANOVA with Tukey’s test