Fig. 10

Hsa-miR-146a-5p involvement in the regulation of APTG-CM-induced osteogenic differentiation of PDLSCs. PDLSCs transduced with recombinant lentivirus miR-146-5p were examined by phase contrast microscopy (A), fluorescent microscopy (B) and merged images (C). MiR-146a-5p expression was validated by qRT-PCR, and the results showed that the expression of miR-146a-5p in cells was significantly increased with Lenti -miR-146-5p transduction and decreased with lentivirus anti-miR-146-5p transduction. The expression of OCN, ALP and BSP were detected by RT-qPCR. The results showed that the expression of ALP and BSP was increased in cells transduced with Lenti-miR-126 after 7, 14, 21 days in culture. The results also showed the expression of BSP was decreased in cells transduced with Lenti-anti-miR-126 after 7, 14, 21 days in culture, and the expression of ALP was decreased after 7 days in culture. The expression of OCN was increased and decreased in cells transduced with Lenti-miR-146-5p and Lenti-anti-miR-146-5p after 21 days in culture, respectively (E–G). Lenti-miR-146-5p-treated groups showed higher ALP activity than control-treated groups, while Lenti-anti-miR-146-5p-treated groups showed lower ALP activity than control-treated groups (H). Data were as mean control-treated *P < 0.05; **P < 0.01; ***P < 0.001