Fig. 6From: Promotion effect of apical tooth germ cell-conditioned medium on osteoblastic differentiation of periodontal ligament stem cells through regulating miR-146a-5pMineralization formation investigated by Alizerin Red Solution staining. PDLSCs cultured in complete MSC Nutristem medium supplied with 2 mmol/l β-glycerophoshate for a period of 2 (A) and 3 weeks (C) showed few mineralized nodules. PDLSCs co-cultured with APTG-CM for a period of 2 (B) and 3 weeks (D) showed the abundance of nodules. Nodule areas were significantly higher in APTG-CM-induced PDLSCs than non-induced PDLSCs (E). *P < 0.05; ** P < 0.01; ***P < 0.001. Scale bar: 100 μm (A–D)Back to article page