Fig. 4

Identification of DNA repair-related genes controlled by metastatic-specific enhancers in OSCC. A, identification of differentially expressed DNA repair-related genes between cluster 2 and cluster 3 were performed based on TCGA. The cut-off values for differentially expressed genes were P < 0.05 and |log2 fold change|>1. B, overlapping analysis of upregulated genes controlled by enhancers in primary OSCC and metastatic OSCC. Enhancers of DNA repair-related genes in metastatic OSCC and primary OSCC were screened using H3K27ac ChIP-seq data of GSE120634. DNA repair-related gene located closest to an enhancer on the genome was defined as an enhancer-controlled DNA repair-related gene. C, H3K27ac peaks of metastatic-specific enhancers of DNA repair-related genes (n = 17), primary-specific enhancers of DNA repair-related genes (n = 69), and enhancers of DNA repair-related genes common to both metastatic OSCC and primary OSCC (n = 36) were shown in heatmap and aggregation plots